Portal de investigação
Tet3 Deletion in Adult Brain Neurons of Female Mice Results in Anxiety-like Behavior and Cognitive Impairments
Autores da FMUP
Participantes de fora da FMUP
- Antunes, C
- Da Silva, JD
- Guerra Gomes, S
- Alves, ND
- Loureiro Campos, E
- Pinto, L
Unidades de investigação
Abstract
TET enzymes (TET1-3) are dioxygenases that oxidize 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and are involved in the DNA demethylation process. In line with the observed 5hmC abundance in the brain, Tet genes are highly transcribed, with Tet3 being the predominant member. We have previously shown that Tet3 conditional deletion in the brain of male mice was associated with anxiety-like behavior and impairment in hippocampal-dependent spatial orientation. In the current study, we addressed the role of Tet3 in female mice and its impact on behavior, using in vivo conditional and inducible deletion from post-mitotic neurons. Our results indicate that conditional and inducible deletion of Tet3 in female mice increases anxiety-like behavior and impairs both spatial orientation and short-term memory. At the molecular level, we identified upregulation of immediate-early genes, particularly Npas4, in both the dorsal and ventral hippocampus and in the prefrontal cortex. This study shows that deletion of Tet3 in female mice differentially affects behavioral dimensions as opposed to Tet3 deletion in males, highlighting the importance of studying both sexes in behavioral studies. Moreover, it contributes to expand the knowledge on the role of epigenetic regulators in brain function and behavioral outcome.
Dados da publicação
- ISSN/ISSNe:
- 0893-7648, 1559-1182
- Tipo:
- Article
- Páginas:
- 4892-4901
- Link para outro recurso:
- www.scopus.com
Molecular Neurobiology Humana Press
Citações Recebidas na Web of Science: 3
Citações Recebidas na Scopus: 7
Documentos
- Não há documentos
Filiações
Keywords
- TET enzymes; 5hmC; Anxiety; Epigenetics; DNA methylation; Brain
Campos de estudo
Financiamento
Proyectos asociados
Epigenetic and transcriptional analysis ofTSC2 in leukocyte DNA from tuberous sclerosis patients.
Investigador Principal: Cristina Joana Moreira Marques
Estudo Observacional Académico (Epigenetic TSA) . Tuberous Sclerosis Association . 2019
Citar a publicação
Antunes C,Da JD,Guerra S,Alves ND,Loureiro E,Pinto L,Marques J. <i>Tet3</i> Deletion in Adult Brain Neurons of Female Mice Results in Anxiety-like Behavior and Cognitive Impairments. Mol. Neurobiol. 2022. 59. (8):p. 4892-4901. IF:5,100. (2).
