Valorizing Coffee Silverskin Based on Its Phytochemicals and Antidiabetic Potential: From Lab to a Pilot Scale

Data de publicação:

Autores da FMUP

  • Maria De Fátima Moreira Martel

    Autor

Participantes de fora da FMUP

  • Peixoto, JAB
  • Andrade, N
  • Machado, S
  • Costa, ASG
  • Puga, H
  • Oliveira, MBPP
  • Alves, RC

Unidades de investigação

Abstract

This study investigates the possibility of valorizing coffee silverskin through the recovery of its bioactive compounds using a sustainable extraction method that could be industrially applied. For that, aqueous extracts were prepared using ultrasonic-assisted extraction (laboratorial scale) and, for comparison, a scale-up of the process was developed using the Multi-frequency Multimode Modulated technology. A concentration procedure at the pilot scale was also tested. The three types of extracts obtained were characterized regarding caffeine and chlorogenic acids contents, and the effects on intestinal glucose and fructose uptake (including sugar transporters expression) in human intestinal epithelial (Caco-2) cells were ascertained. The phytochemical contents of the extracts prepared at the laboratory and pilot scale were comparable (caffeine: 27.7 vs. 29.6 mg/g freeze-dried extract; 3-, 4-, and 5-caffeoylquinic acids: 0.19 vs. 0.31, 0.15 vs. 0.42, and 1.04 vs. 1.98 mg/g, respectively; 4- and 5-feruloylquinic acids: 0.39 vs. 0.43 and 1.05 vs. 1.32 mg/g, respectively). Slight differences were noticed according to the extracts preparation steps, but in general, all the extracts promoted significant inhibitions of [1,2-H-3(N)]-deoxy-D-glucose and C-14-D-fructose uptake, which resulted mainly from a decrease on the facilitative glucose transporter 2 (GLUT2) and sodium-glucose linked transporter 1 (SGLT1) genes expression but not on the expression of the facilitative glucose transporter 5 (GLUT5) gene. Moreover, a synergistic effect of caffeine and 5-caffeoylquinic acid on sugars uptake was found. The results clearly show that the Multi-frequency Multimode Modulated technology is a viable option to be applied at an industrial level to recover bioactive components from silverskin and obtain extracts with antidiabetic potential that could be used to develop functional food products or dietary supplements.

Dados da publicação

ISSN/ISSNe:
2304-8158, 2304-8158

Foods  Multidisciplinary Digital Publishing Institute (MDPI)

Tipo:
Article
Páginas:
1671-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 5

Citações Recebidas na Scopus: 7

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Keywords

  • coffee by-product; sustainability; health benefits; innovative applications; scale-up; green technology

Financiamento

Proyectos asociados

The role of EGCG in breast cancer prevention and therapy

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico (EGCG) . 2020

Intestinal fructose absorption: regulation and relation to human diseases

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

The role of the glutamine transporter ASCT2 in antineoplastic therapy

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

The effect of dietary polyphenols upon the intestinal absorption of fructose: relevance for metabolic syndrome

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

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