Portal de investigação
The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent
Autores da FMUP
Participantes de fora da FMUP
- Andrade, N
- Cardoso, B.
- Meireles, C
- Pinto, V
- Paiva, J.
Unidades de investigação
Abstract
Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12A(DMBA)) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, H-3-DG uptake and lactic acid production in both cell lines. The stimulatory effects of insulin upon cell proliferation and H-3-DG uptake were hampered by rapamycin, LY294001 and BAY-876, in both cell lines. In conclusion, hyperinsulinemia, one important characteristic of T2DM, contributes to the initiation of breast cancer by a PI3K- and mTOR-dependent mechanism involving increased GLUT1-mediated glucose uptake. Significance The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent.
Dados da publicação
- ISSN/ISSNe:
- 0263-6484, 1099-0844
- Tipo:
- Article
- Páginas:
- 127-137
- DOI:
- 10.1002/cbf.3681
- Link para outro recurso:
- www.scopus.com
Cell Biochemistry and Function John Wiley and Sons Ltd
Citações Recebidas na Scopus: 2
Documentos
- Não há documentos
Filiações
Keywords
- breast cancer; DMBA; GLUT1; insulin; mTOR; PI3K
Financiamento
Proyectos asociados
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The effect of dietary polyphenols upon the intestinal absorption of fructose: relevance for metabolic syndrome
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Estudo Clínico Académico . 2020
Citar a publicação
Silva C,Andrade N,Guimaraes JT,Cardoso B,Meireles C,Pinto V,Paiva J,Martel F. The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent. Cell Biochem. Funct. 2022. 40. (2):p. 127-137. IF:3,600. (3).
