The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent

Data de publicação: Data Ahead of Print:

Autores da FMUP

  • Cláudia Raquel Santos Silva

    Autor

  • João Tiago De Sousa Pinto Guimarães

    Autor

  • Maria De Fátima Moreira Martel

    Autor

Participantes de fora da FMUP

  • Andrade, N
  • Cardoso, B.
  • Meireles, C
  • Pinto, V
  • Paiva, J.

Unidades de investigação

Abstract

Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12A(DMBA)) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, H-3-DG uptake and lactic acid production in both cell lines. The stimulatory effects of insulin upon cell proliferation and H-3-DG uptake were hampered by rapamycin, LY294001 and BAY-876, in both cell lines. In conclusion, hyperinsulinemia, one important characteristic of T2DM, contributes to the initiation of breast cancer by a PI3K- and mTOR-dependent mechanism involving increased GLUT1-mediated glucose uptake. Significance The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent.

Dados da publicação

ISSN/ISSNe:
0263-6484, 1099-0844

Cell Biochemistry and Function  John Wiley and Sons Ltd

Tipo:
Article
Páginas:
127-137
Link para outro recurso:
www.scopus.com

Citações Recebidas na Scopus: 2

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Keywords

  • breast cancer; DMBA; GLUT1; insulin; mTOR; PI3K

Financiamento

Proyectos asociados

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Estudo Clínico Académico . 2020

The role of the glutamine transporter ASCT2 in antineoplastic therapy

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Estudo Clínico Académico . 2020

The effect of dietary polyphenols upon the intestinal absorption of fructose: relevance for metabolic syndrome

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Estudo Clínico Académico . 2020

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