Exploring Gunnera tinctoria: From Nutritional and Anti-Tumoral Properties to Phytosome Development Following Structural Arrangement Based on Molecular Docking

Data de publicação:

Autores da FMUP

  • Cláudia Raquel Santos Silva

    Autor

  • Maria De Fátima Moreira Martel

    Autor

Participantes de fora da FMUP

  • Fathi, F
  • Ebrahimi, SN
  • Valadao, AIG
  • Andrade, N
  • Costa, ASG
  • Fathi, A
  • Salehi, P
  • Alves, RC
  • Oliveira, MBPP

Unidades de investigação

Abstract

Gunnera tinctoria, an underexplored invasive plant found in Azores, Portugal, was studied regarding its nutritional, antioxidant, and antitumoral properties. Higher antioxidant activity was found in baby leaves, followed by adult leaves and inflorescences. A phenolic fraction of the plant was enriched using adsorbent resin column chromatography (Diaion(TM) HP20LX, and Relite EXA90). Antitumoral effects were observed with the enriched fractions in breast (MCF-7) and pancreatic (AsPC-1) cancer cell lines, being more pronounced in the latter. To improve protection and membrane absorption rates of phenolic compounds, nano-phytosomes and cholesterol-conjugated phytosomes coated with natural polymers were loaded with the enriched fraction. The particles were characterized, and their physiochemical properties were evaluated and compared. All samples presented anionic charge and nanometer size in relation to the inner layer and micrometer size regarding the external layers. In addition, the molecular arrangement of phenolics within both types of phytosomes were studied for the first time by molecular docking. Polarity and molecular size were key factors on the molecular arrangement of the lipid bilayer. In conclusion, G. tinctoria showed to be an interesting source of nutrients and phenolic compounds with anti-tumoral potential. Moreover, phytosome loading with these compounds can increase their stability and bioavailability having in view future applications.

Dados da publicação

ISSN/ISSNe:
1420-3049, 1420-3049

Molecules  Multidisciplinary Digital Publishing Institute (MDPI)

Tipo:
Article
Páginas:
5935-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 5

Citações Recebidas na Scopus: 10

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Keywords

  • bioactive compounds; phytosome; secondary layer; molecular docking; bilayer arrangement

Financiamento

Proyectos asociados

The role of EGCG in breast cancer prevention and therapy

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico (EGCG) . 2020

Intestinal fructose absorption: regulation and relation to human diseases

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

The role of the glutamine transporter ASCT2 in antineoplastic therapy

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

The effect of dietary polyphenols upon the intestinal absorption of fructose: relevance for metabolic syndrome

Investigador Principal: Maria de Fátima Moreira Martel

Estudo Clínico Académico . 2020

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