Portal de investigação
MicroRNA Dysregulation in the Gastric Carcinogenesis Cascade: Can We Anticipate Its Role in Individualized Care?
Autores da FMUP
Participantes de fora da FMUP
- Pita, I
- Dias, F
- Teixeira, AL
- Nogueira, I
- Medeiros, R.
Unidades de investigação
Abstract
Background: Gastric carcinogenesis progresses from normal mucosa, atrophic/metaplastic gastritis, and dysplasia to adenocarcinoma. MicroRNAs (miRNAs) regulate DNA expression and have been implicated; however, their role is not fully established. Aims: The aim of this study was to characterize plasma and tissue expression of several miRNAs in gastric carcinogenesis stages. Methods: Single-center cross-sectional study in 64 patients: 19 controls (normal mucosa); 15 with extensive atrophic/metaplastic gastritis; and 30 with early gastric neoplasia (EGN). Seven miRNAs (miR-21, miR-146a, miR-181b, miR-370, miR-375, miR 181b, and miR-490) were quantified by real time-qPCR in peripheral blood and endoscopic biopsy samples. Results: We found a significant upregulation of miR-181b, miR-490, and miR-21 in the EGN mucosa (overexpression 2-14-times higher than controls). We observed a significant underexpression of miR-146a and miR-370 in atrophic/metaplastic gastritis (86 and 66% decrease, p = 0.008 and p = 0.001) and in EGN (89 and 62% reduction, p = 0.034 and p = 0.032) compared with controls. There were no differences between lesions and nonneoplastic mucosa and no dysregulation of plasma miRNAs. Conclusion: We found significant dysregulation of 5 miRNAs in gastric carcinogenesis, suggesting a tumor suppressor role for miR-146a and miR-370 and oncogenic potential for miR-21, miR-181, and miR-490. These changes happen diffusely in the gastric mucosa, suggesting a high-risk field defect, which may influence these patients' surveillance.
© 2021 S. Karger AG, Basel.
Dados da publicação
- ISSN/ISSNe:
- 1423-0291, 1015-2008
- Tipo:
- Article
- Páginas:
- 338-350
- DOI:
- 10.1159/000515548
- Link para outro recurso:
- www.scopus.com
Pathobiology S. Karger AG
Citações Recebidas na Scopus: 1
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Filiações
Keywords
- Gastric cancer; Gastric carcinogenesis; Gastric preneoplastic conditions; microRNAs
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Citar a publicação
Pita I,Lib?nio D,Dias F,Teixeira AL,Nogueira I,Medeiros R,Dinis M,Pimentel P. MicroRNA Dysregulation in the Gastric Carcinogenesis Cascade: Can We Anticipate Its Role in Individualized Care?. Pathobiology. 2021. 88. (5):p. 338-350. IF:3,916. (2).
