Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue

Data de publicação:

Autores da FMUP

  • Ricardo José Moreira Horta Oliveira

    Autor

Participantes de fora da FMUP

  • Freitas-Ribeiro, S
  • Diogo, GS
  • Oliveira, C
  • Martins, A
  • Silva, TH
  • Jarnalo, M
  • Reis, RL
  • Pirraco, RP

Unidades de investigação

Abstract

The successful integration of transplanted three-dimensional tissue engineering (TE) constructs depends greatly on their rapid vascularization. Therefore, it is essential to address this vascularization issue in the initial design of constructs for perfused tissues. Two of the most important variables in this regard are scaffold composition and cell sourcing. Collagens with marine origins overcome some issues associated with mammal-derived collagen while maintaining their advantages in terms of biocompatibility. Concurrently, the freshly isolated stromal vascular fraction (SVF) of adipose tissue has been proposed as an advantageous cell fraction for vascularization purposes due to its highly angiogenic properties, allowing extrinsic angiogenic growth factor-free vascularization strategies for TE applications. In this study, we aimed at understanding whether marine collagen 3D matrices could support cryopreserved human SVF in maintaining intrinsic angiogenic properties observed for fresh SVF. For this, cryopreserved human SVF was seeded on blue shark collagen sponges and cultured up to 7 days in a basal medium. The secretome profile of several angiogenesis-related factors was studied throughout culture times and correlated with the expression pattern of CD31 and CD146, which showed the formation of a prevascular network. Upon in ovo implantation, increased vessel recruitment was observed in prevascularized sponges when compared with sponges without SVF cells. Immunohistochemistry for CD31 demonstrated the improved integration of prevascularized sponges within chick chorioalantoic membrane (CAM) tissues, while in situ hybridization showed human cells lining blood vessels. These results demonstrate the potential of using cryopreserved SVF combined with marine collagen as a streamlined approach to improve the vascularization of TE constructs.

Dados da publicação

ISSN/ISSNe:
1660-3397, 1660-3397

Marine Drugs  Multidisciplinary Digital Publishing Institute (MDPI)

Tipo:
Article
Páginas:
623-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 2

Citações Recebidas na Scopus: 3

Documentos

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Keywords

  • stromal vascular fraction; vascularization; blue shark skin collagen; 3D constructs

Financiamento

Proyectos asociados

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Estudo Clínico Académico . 2022

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