Portal de investigação
Pericardial Fluid Accumulates microRNAs That Regulate Heart Fibrosis after Myocardial Infarction
Data de publicação:
Data Ahead of Print:
Autores da FMUP
Participantes de fora da FMUP
- Silva, ED
- Pereira-Sousa, D
- Ribeiro-Costa, F
- Enguita, FJ
- Gomes, RN
- Dias-Ferreira, J
- Pereira, C
- Castanheira, A
- Pinto-do-O, P
- Nascimento, DS
Unidades de investigação
Abstract
Pericardial fluid (PF) has been suggested as a reservoir of molecular targets that can be modulated for efficient repair after myocardial infarction (MI). Here, we set out to address the content of this biofluid after MI, namely in terms of microRNAs (miRs) that are important modulators of the cardiac pathological response. PF was collected during coronary artery bypass grafting (CABG) from two MI cohorts, patients with non-ST-segment elevation MI (NSTEMI) and patients with ST-segment elevation MI (STEMI), and a control group composed of patients with stable angina and without previous history of MI. The PF miR content was analyzed by small RNA sequencing, and its biological effect was assessed on human cardiac fibroblasts. PF accumulates fibrotic and inflammatory molecules in STEMI patients, namely causing the soluble suppression of tumorigenicity 2 (ST-2), which inversely correlates with the left ventricle ejection fraction. Although the PF of the three patient groups induce similar levels of fibroblast-to-myofibroblast activation in vitro, RNA sequencing revealed that PF from STEMI patients is particularly enriched not only in pro-fibrotic miRs but also anti-fibrotic miRs. Among those, miR-22-3p was herein found to inhibit TGF-beta-induced human cardiac fibroblast activation in vitro. PF constitutes an attractive source for screening diagnostic/prognostic miRs and for unveiling novel therapeutic targets in cardiac fibrosis.
Dados da publicação
- ISSN/ISSNe:
- 1661-6596, 1661-6596
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/ijms25158329
- Link para outro recurso:
- www.scopus.com
International Journal of Molecular Sciences Multidisciplinary Digital Publishing Institute (MDPI)
Documentos
- Não há documentos
Filiações
Keywords
- myocardial infarction; pericardial fluid; fibrosis; miRNAs; miR-22-3p; cardiac fibroblasts
Financiamento
Proyectos asociados
Dapagliflozin, Spironolactone or Both for HFpEF (SOGALDI-PEF) - NCT05676684
Investigador Principal: João Pedro Melo Marques Pinho Ferreira
Ensaio Clínico Académico (SOGALDI-PEF) . AstraZeneca . 2022
The Canadian CABG or PCI in Patients with Ischemic Cardiomyopathy Trial (STICH 3C) - NCT05427370
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Ensaio Clínico Académico (STICH 3C) . Cornell University . 2023
Estudo do tratamento da doença valvular aórtica.
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Observacional Académico (AORTA) . UniC . 2019
Eficácia da transposição de um pedículo adiposo pericárdico sobre o enfarte de miocárdio em pacientes (ensaio AGTP II)
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Ensaio Clínico Académico (Ensaio AGTP II) . 2019
Aortic valve surgery with stented versus stentless bioprosthesis in patients with active infective endocarditis
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico (Endocarditis) . 2023
Early Dual Antiplatelet Therapy versus Aspirin Monotherapy after Coronary Artery Bypass Surgery: survival and safety outcomes
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico (Bypass) . 2020
Early and Midterm Outcomes following Aortic Valve Replacement with Mechanical versus Bioprosthetic Valves in Patients aged 50 to 70 Years
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico (Aortic Valve Replacemen) . 2020
Efeito da terapêutica ß-bloqueadora pré-operatória após cirurgia de revascularização do miocárdio: sobrevida e complicações pós-operatórias
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2021
"Aortic valve surgery in patients with infective endocarditis: mid-term follow-up of patients treated with the St. Jude medical trifecta™ valve"
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2022
Contemporary Aortic Valve Replacement Therapies: Follow-Up And Lifelong Management
Investigador Principal: Rui João Gonçalves Cerqueira
Estudo Clínico Académico . 2022
Long-term survival after coronary artery bypass grafting: the relevance of surgical procedures analysed by propensity score methods.
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico (Arrtery bypass) . 2022
Abdominal Aortic Aneurysm - The Portuguese Case: Specificities and Consequences
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2022
New Insights into Epidemiology and Pathophysiology of Abdominal Aortic Aneurysms
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2021
Impacto clínico e hemodinâmico da substituição cirúrgica da válvula aórtica por biopróteses de última geração
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2020
Condicionamento isquémico cardíaco remoto como adjuvante da revascularização miocárdia na doença coronário aguda
Investigador Principal: Joaquim Adelino Correia Ferreira Leite Moreira
Estudo Clínico Académico . 2020
Citar a publicação
Silva ED,Pereira D,Ribeiro F,Cerqueira R,Enguita FJ,Gomes RN,Dias J,Pereira C,Castanheira A,Pinto P,leite AF,Nascimento DS. Pericardial Fluid Accumulates microRNAs That Regulate Heart Fibrosis after Myocardial Infarction. Int. J. Mol. Sci. 2024. 25. (15):8329. IF:5,600. (1).
