The extent of postpartum cardiac reverse remodeling is reflected in urine proteome

Data de publicação: 01/01/2024 Data Ahead of Print: 27/06/2024

Autores da FMUP

• Joaquim Adelino Correia Ferreira Leite Moreira

  Autor

• António Sousa Barros

  Autor

Participantes de fora da FMUP

• Ferreira A.F.
• Trindade F.
• Azevedo M.J.
• Morais J.
• Douché T.
• Diaz S.O.
• Saraiva F.A.
• Sousa C.
• Machado A.P.
• Matondo M.
• Ramalho C.
• Vitorino R.
• Falcão-Pires I.

Unidades de investigação

• Cirurgia e Fisiologia

• Laboratório Associado RISE- Rede de Investigação em Saúde

  

  Investigador

  Fernando Carlos De Landér Schmitt

• RISE-Health

  

  Investigador

  Fernando Carlos De Landér Schmitt

Abstract

The association of postpartum cardiac reverse remodeling (RR) with urinary proteome, particularly in pregnant women with cardiovascular (CV) risk factors who show long-term increased risk of cardiovascular disease and mortality is unknown. We aim to profile the urinary proteome in pregnant women with/without CV risk factors to identify proteins associated with postpartum RR. Our study included a prospective cohort of 32 healthy and 27 obese and/or hypertensive and/or diabetic pregnant women who underwent transthoracic echocardiography, pulse-wave-velocity, and urine collection at the 3rd trimester and 6 months postpartum. Shotgun HPLC–MS/MS profiled proteins. Generalized linear mixed-effects models were used to identify associations between urinary proteins and left ventricle mass (LVM), a surrogate of RR. An increase in arterial stiffness was documented from 3rd trimester to 6 months after delivery, being significantly elevated in women with CV risk factors. In addition, the presence of at least one CV risk factor was associated with worse LVM RR. We identified 6 and 11 proteins associated with high and low LVM regression, respectively. These proteins were functionally linked with insulin-like growth factor (IGF) transport and uptake regulation by IGF binding-proteins, platelet activation, signaling and aggregation and the immune system’s activity. The concentration of IGF-1 in urine samples was associated with low LVM regression after delivery. Urinary proteome showed a predicting potential for identifying pregnant women with incomplete postpartum RR. © The Author(s) 2024.

© 2024. The Author(s).

Keywords

• Adult; Biomarkers; Echocardiography; Female; Humans; Postpartum Period; Pregnancy; Prospective Studies; Proteome; Risk Factors; Vascular Stiffness; Ventricular Remodeling; biological marker; proteome; adult; arterial stiffness; echocardiography; female; heart ventricle remodeling; human; pregnancy; prospective study; puerperium; risk factor; urine