Effects of 2',6'-dihydroxy-4'-methoxydihidrochalcone on innate inflammatory response.

Data de publicação: Data Ahead of Print:

Autores da FMUP

  • Rui Manuel Lopes Nunes

    Autor

Participantes de fora da FMUP

  • Cerutti ML
  • Benvenutti L
  • da Silva SR
  • Barauna SC
  • de Souza MM
  • Malheiros Â
  • Lacava L
  • Quintão NLM
  • Santin JR

Unidades de investigação

Abstract

Chalcones present potential therapeutic activities reported on literature, which led us to evaluate the anti-inflammatory effects and the acute toxicity of 2',6'-dihydroxy-4'-methoxydihydrochalcone (DHMDC) using in vitro and in vivo models. The anti-inflammatory activity was firstly in vitro investigated using macrophages (RAW 264.7) and neutrophils previously treated with DHMCD activated with lipopolysaccharide (LPS). Nitrite, IL-1ß, and TNF levels were measured in the macrophage culture supernatant, and the adhesion molecule expression (CD62L, CD49D, and CD18) was evaluated in neutrophils. Then, carrageenan-induced inflammation was performed in the subcutaneous tissue of male Swiss mice. Leukocyte migration and histological analysis were performed in the pouches. Toxicological studies were carried out on female Swiss mice (600 mg/kg) through biochemical parameters and histopathological analysis. In vitro, the DHMCD significantly reduced the IL-1ß, TNF, and nitrite levels. The DHMCD was also able to modulate the percentage of positive neutrophils for CD62L, without modifying the expression of CD18 or CD49d. In vivo, DHMCD (3 mg/kg, p.o.) significantly reduced neutrophil migration to inflammatory exudate and subcutaneous tissue. No evidence of toxic effect was observed considering the biochemical parameters and histopathological analysis of liver and kidney. Together, the obtained data shows that DHMCD presents anti-inflammatory activity by modulating the macrophage inflammatory protein secretion and also by blocking the CD62L cleavage in neutrophils. Furthermore, there was not any evidence of toxic effect in acute toxicological analysis.

Dados da publicação

ISSN/ISSNe:
0028-1298, 1432-1912

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY  Springer Verlag

Tipo:
Article
Páginas:
2061-2072

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Keywords

  • Adhesion molecules; Chalcone; Inflammation; Macrophages; Mice; Neutrophil

Proyectos asociados

Ameaça aos princípios bioéticos na pratica médica no Brasil: Duas Décadas dos Processos ético-profissionais (1988-2008)

Investigador Principal: Rui Manuel Lopes Nunes

Estudo Clínico Académico . 2019

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