Generation of 3D melanoma models using an assembloid-based approach

Data de publicação: Data Ahead of Print:

Autores da FMUP

  • Ricardo José Moreira Horta Oliveira

    Autor

Participantes de fora da FMUP

  • Rodrigues, DB
  • Moreira, HR
  • Jarnalo, M
  • Marques, AP
  • Reis, RL
  • Pirraco, RP

Unidades de investigação

Abstract

While 3D tumor models have greatly evolved over the past years, there is still a strong requirement for more biosimilar models which are capable of recapitulating cellular crosstalk within the tumor microenvironment while equally displaying representative levels of tumor aggressiveness and invasion. Herein, we disclose an assembloid melanoma model based on the fusion of individual stromal multicellular spheroids (MCSs). In contrast to more traditional tumor models, we show that it is possible to develop self-organizing, heterotypic melanoma models where tumor cells present stem -cell like features like upregulated pluripotency master regulators SOX2, POU5F1 and NANOG. Additionally, these assembloids display high levels of invasiveness while embedded in 3D matrices as evidenced by stromal cell promotion of melanoma cell invasion via metalloproteinase production. Furthermore, sensitivity to anticancer drug doxorubicin was demonstrated for the melanoma assembloid model. These findings suggest that melanoma assembloids may play a significant role in the field of 3D cancer models as they more closely mimic the tumor microenvironment when compared to more traditional MCSs, opening the doors to a better understanding of the role of tumor microenvironment in supporting tumor progression.

Dados da publicação

ISSN/ISSNe:
1742-7061, 1878-7568

Acta Biomaterialia  Elsevier BV

Tipo:
Article
Páginas:
93-110
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 2

Citações Recebidas na Scopus: 2

Documentos

  • Não há documentos

Métricas

Filiações mostrar / ocultar

Keywords

  • Assembloids; Tumor models; Melanoma; Spheroids; 3D models

Financiamento

Proyectos asociados

Tissue Engineering in Plastic Surgery - What Has Been Done

Investigador Principal: Ricardo José Moreira Horta Oliveira

Estudo Clínico Académico (Plastic Surgery) . 2020

Breast Implant-Associated Anaplastic Large Cell Lymphoma - the epidemiological profile in Portugal: a cross-sectional study

Investigador Principal: Ricardo José Moreira Horta Oliveira

Estudo Clínico Académico (Breast Implan) . 2020

The efficacy of combined adjuvant radiotherapy with surgical excision in the treatment of keloids.

Investigador Principal: Ricardo José Moreira Horta Oliveira

Ensaio Clínico Académico (Keloids) . 2020

The Relation between Human Characteristics with the Angiogenic and Proliferative Potential of the Stromal Vascular Cells obtained from Subcutaneous Adipose Tissue

Investigador Principal: Ricardo José Moreira Horta Oliveira

Estudo Clínico Académico . 2021

Spontaneous keloids: a rare entity

Investigador Principal: Ricardo José Moreira Horta Oliveira

Estudo Clínico Académico . 2022

A ruler for abdominoplasty preoperative markings: the potential of best scar symmetry

Investigador Principal: Ricardo José Moreira Horta Oliveira

Estudo Clínico Académico . 2020

Citar a publicação

Partilhar a publicação