Portal de investigação
Generation of 3D melanoma models using an assembloid-based approach
Data de publicação:
Data Ahead of Print:
Autores da FMUP
Participantes de fora da FMUP
- Rodrigues, DB
- Moreira, HR
- Jarnalo, M
- Marques, AP
- Reis, RL
- Pirraco, RP
Unidades de investigação
Abstract
While 3D tumor models have greatly evolved over the past years, there is still a strong requirement for more biosimilar models which are capable of recapitulating cellular crosstalk within the tumor microenvironment while equally displaying representative levels of tumor aggressiveness and invasion. Herein, we disclose an assembloid melanoma model based on the fusion of individual stromal multicellular spheroids (MCSs). In contrast to more traditional tumor models, we show that it is possible to develop self-organizing, heterotypic melanoma models where tumor cells present stem -cell like features like upregulated pluripotency master regulators SOX2, POU5F1 and NANOG. Additionally, these assembloids display high levels of invasiveness while embedded in 3D matrices as evidenced by stromal cell promotion of melanoma cell invasion via metalloproteinase production. Furthermore, sensitivity to anticancer drug doxorubicin was demonstrated for the melanoma assembloid model. These findings suggest that melanoma assembloids may play a significant role in the field of 3D cancer models as they more closely mimic the tumor microenvironment when compared to more traditional MCSs, opening the doors to a better understanding of the role of tumor microenvironment in supporting tumor progression.
Dados da publicação
- ISSN/ISSNe:
- 1742-7061, 1878-7568
- Tipo:
- Article
- Páginas:
- 93-110
- Link para outro recurso:
- www.scopus.com
Acta Biomaterialia Elsevier BV
Citações Recebidas na Web of Science: 2
Citações Recebidas na Scopus: 2
Documentos
- Não há documentos
Filiações
Keywords
- Assembloids; Tumor models; Melanoma; Spheroids; 3D models
Financiamento
Proyectos asociados
Tissue Engineering in Plastic Surgery - What Has Been Done
Investigador Principal: Ricardo José Moreira Horta Oliveira
Estudo Clínico Académico (Plastic Surgery) . 2020
Breast Implant-Associated Anaplastic Large Cell Lymphoma - the epidemiological profile in Portugal: a cross-sectional study
Investigador Principal: Ricardo José Moreira Horta Oliveira
Estudo Clínico Académico (Breast Implan) . 2020
The efficacy of combined adjuvant radiotherapy with surgical excision in the treatment of keloids.
Investigador Principal: Ricardo José Moreira Horta Oliveira
Ensaio Clínico Académico (Keloids) . 2020
The Relation between Human Characteristics with the Angiogenic and Proliferative Potential of the Stromal Vascular Cells obtained from Subcutaneous Adipose Tissue
Investigador Principal: Ricardo José Moreira Horta Oliveira
Estudo Clínico Académico . 2021
Spontaneous keloids: a rare entity
Investigador Principal: Ricardo José Moreira Horta Oliveira
Estudo Clínico Académico . 2022
A ruler for abdominoplasty preoperative markings: the potential of best scar symmetry
Investigador Principal: Ricardo José Moreira Horta Oliveira
Estudo Clínico Académico . 2020
Citar a publicação
Rodrigues DB,Moreira HR,Jarnalo M,Horta R,Marques AP,Reis RL,Pirraco RP. Generation of 3D melanoma models using an assembloid-based approach. Acta Biomater. 2024. 178. p. 93-110. IF:9,700. (1).
