Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue

Autores da FMUP

• Gil Filipe Ramada Faria

  Autor

• Maria Da Conceição Costa Pinho Calhau

  Autor

Participantes de fora da FMUP

• Sousa, Sara
• Pestana, Diogo
• Vasconcelos, Fernando
• Delerue-Matos, Cristina
• Domingues, Valentina Fernandes

Unidades de investigação

• Cirurgia e Fisiologia

Abstract

Synthetic musks and organophosphorus pesticides represent a potential risk to the human health since exposure can lead to distinct types of carcinogenesis and endocrine disorders. These are lipophilic compounds as such, prone to deposit and persist in fat tissues, mainly in adipose tissue. Very few studies have reported on the occurrence and accumulation profile of these contaminants in human adipose tissue. Analytical methods for the detection and quantification of synthetic musks and organophosphorus pesticides in adipose tissue are lacking. In this study, the efficacy of different extraction with ultrasonic homogenizer and dispersive solid-phase extraction (d-SPE) clean-up methods were evaluated inhuman adipose tissue. The relative sample clean-up was assessed by measurement of total lipid content. The quantification of four synthetic musks and six organophosphorus pesticides were performed by gas chromatography (GC) mass spectrometry (MS) and flame photometric detection (FPD), respectively. The d-SPE clean-up with 50 mg PSA, 150 mg MgSO4, 100 mg C18EC and 50 mg Z-Sep provided the most effective clean-up, removing the greatest amount of interfering substances including lipids and simultaneously ensuring good chromatographic separation and recoveries. Method detection limits were between 4 to 9 ng/g for synthetic musk and 1 to 7 ng/g for organophosphorus pesticides in adiposet issue. The proposed method was applied to adipose tissue of obese patients and positive samples were confirmed with GC tandem mass spectrometry. Galaxolide was found in all the samples tested with concentrations ranging from 0.08 to 0.5 mu g/g of adipose tissue. No other synthetic musk studied was detected. Organophosphorus pesticides were not found in the analysed samples. The developed analytical procedures were successful and can easily be applied to biomonitoring these compounds in human adipose tissue. (C) 2020 Elsevier B.V. All rights reserved.

Keywords

• adipose tissue; GC-FPD; GC-MS/MS; GC-MS; organophosphorus pesticides; synthetic musksa