Portal de investigação
The mitochondrial enzyme FAHD1 regulates complex II activity in breast cancer cells and is indispensable for basal BT-20 cells in vitro.
Data de publicação:
Data Ahead of Print:
Autores da FMUP
Participantes de fora da FMUP
- Holzknecht M
- Guerrero-Navarro L
- Petit M
- Albertini E
- Damisch E
- Simonini A
- Parson W
- Fiegl H
- Weiss A
- Jansen-Duerr P
Unidades de investigação
Abstract
The mitochondrial enzyme fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) was identified to be upregulated in breast cancer tissues. Here, we show that FAHD1 is indispensable for the survival of BT-20 cells, representing the basal breast cancer cell type. A lentiviral knock-down of FAHD1 in the breast cancer cell lines MCF-7 and BT-20 results in lower succinate dehydrogenase (complex II) activity. In luminal MCF-7 cells, this leads to reduced proliferation when cultured in medium containing only glutamine as the carbon source. Of note, both cell lines show attenuated protein levels of the enzyme glutaminase (GLS) which activates programmed cell death in BT-20. These findings demonstrate that FAHD1 is crucial for the functionality of complex II in breast cancer cells and acts on glutaminolysis in the mitochondria.
© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Dados da publicação
- ISSN/ISSNe:
- 0014-5793, 1873-3468
- Tipo:
- Article
- Páginas:
- 2781-2794
- Link para outro recurso:
- www.scopus.com
FEBS Letters Wiley-Blackwell
Citações Recebidas na Web of Science: 2
Citações Recebidas na Scopus: 3
Documentos
- Não há documentos
Filiações
Keywords
- FAHD1; MCF-7; TNBC; glutaminase; oxaloacetate; succinate dehydrogenase
Proyectos asociados
Parâmetros que demonstram ter influência na resposta à terapia neoadjuvante em pacientes com cancro da mama: Estado da arte e perspetivas futuras
Investigador Principal: Fernando Carlos de Landér Schmitt
Estudo Clínico Académico . 2021
Citar a publicação
Holzknecht M,Guerrero L,Petit M,Albertini E,Damisch E,Simonini A,Schmitt F,Parson W,Fiegl H,Weiss A,Jansen P. The mitochondrial enzyme FAHD1 regulates complex II activity in breast cancer cells and is indispensable for basal BT-20 cells in vitro. FEBS Lett. 2022. 596. (21):p. 2781-2794. IF:3,500. (2).
