Portal de investigação
Alkaline phosphatase dual-binding sites for collagen dictate cell migration and microvessel assembly in vitro
Autores da FMUP
Participantes de fora da FMUP
- Guerreiro, SG
- Unger, RE
- Cerqueira, NMFSA
- Sartoris, A
- Barbosa, MA
- Granja, PL
- Kirkpatrick, CJ
Unidades de investigação
Abstract
Interactions between cell types, growth factors, and extracellular matrix components involved in angiogenesis are crucial for new vessel formation leading to tissue regeneration. This study investigated whether cocultures of fibroblasts and endothelial cells (ECs; from macro- or microvasculature) play a role in the formation of microvessel-like structures by ECs, as well as modulate fibroblast differentiation and growth factors production (vascular endothelial cell growth factor, basic fibroblast growth factor, active transforming growth factor-beta 1, and interleukin-8), which are important for vessel sprouting and maturation. Data obtained revealed that in vitro coculture systems of fibroblasts and human ECs stimulate collagen synthesis and growth factors production by fibroblasts that ultimately affect the formation and distribution of microvessel-like structures in cell cultures. In this study, areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in cocultures. Molecular docking assays revealed that ALP has two binding positions for collagen, suggesting its impact in collagen proteins' aggregation, cell migration, and microvessel assembly. These findings indicate that bioinformatics and coculture systems are complementary tools for investigating the participation of proteins, like collagen and ALP in angiogenesis.
Dados da publicação
- ISSN/ISSNe:
- 0730-2312, 1097-4644
- Tipo:
- Article
- Páginas:
- 116-129
- DOI:
- 10.1002/jcb.29835
- Link para outro recurso:
- www.scopus.com
Journal of Cellular Biochemistry Wiley-Liss Inc.
Citações Recebidas na Web of Science: 4
Citações Recebidas na Scopus: 4
Documentos
- Não há documentos
Filiações
Keywords
- atherosclerosis; cocultures; endothelial cells; fibroblasts; tissue regeneration; vascular calcification
Financiamento
Proyectos asociados
Effects of Xanthohumol on Metabolic Syndrome Progression (XAN4Health) - NCT03561116
Investigador Principal: Raquel Ângela Silva Soares Lino
Ensaio Clínico Académico (XAN4Health) . TA XAN . 2019
Citar a publicação
Guerreiro SG,Unger RE,Cerqueira N,Sartoris A,Martins MJ,Barbosa MA,Soares R,Granja PL,Kirkpatrick CJ. Alkaline phosphatase dual-binding sites for collagen dictate cell migration and microvessel assembly in vitro. J. Cell. Biochem. 2021. 122. (1):p. 116-129. IF:4,480. (2).
