Alkaline phosphatase dual-binding sites for collagen dictate cell migration and microvessel assembly in vitro

Data de publicação: Data Ahead of Print:

Autores da FMUP

  • Maria João Dos Reis Conceição Martins De Almeida Ribeiro

    Autor

  • Raquel Ângela Silva Soares Lino

    Autor

Participantes de fora da FMUP

  • Guerreiro, SG
  • Unger, RE
  • Cerqueira, NMFSA
  • Sartoris, A
  • Barbosa, MA
  • Granja, PL
  • Kirkpatrick, CJ

Unidades de investigação

Abstract

Interactions between cell types, growth factors, and extracellular matrix components involved in angiogenesis are crucial for new vessel formation leading to tissue regeneration. This study investigated whether cocultures of fibroblasts and endothelial cells (ECs; from macro- or microvasculature) play a role in the formation of microvessel-like structures by ECs, as well as modulate fibroblast differentiation and growth factors production (vascular endothelial cell growth factor, basic fibroblast growth factor, active transforming growth factor-beta 1, and interleukin-8), which are important for vessel sprouting and maturation. Data obtained revealed that in vitro coculture systems of fibroblasts and human ECs stimulate collagen synthesis and growth factors production by fibroblasts that ultimately affect the formation and distribution of microvessel-like structures in cell cultures. In this study, areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in cocultures. Molecular docking assays revealed that ALP has two binding positions for collagen, suggesting its impact in collagen proteins' aggregation, cell migration, and microvessel assembly. These findings indicate that bioinformatics and coculture systems are complementary tools for investigating the participation of proteins, like collagen and ALP in angiogenesis.

Dados da publicação

ISSN/ISSNe:
0730-2312, 1097-4644

Journal of Cellular Biochemistry  Wiley-Liss Inc.

Tipo:
Article
Páginas:
116-129
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 4

Citações Recebidas na Scopus: 4

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Keywords

  • atherosclerosis; cocultures; endothelial cells; fibroblasts; tissue regeneration; vascular calcification

Financiamento

Proyectos asociados

Effects of Xanthohumol on Metabolic Syndrome Progression (XAN4Health) - NCT03561116

Investigador Principal: Raquel Ângela Silva Soares Lino

Ensaio Clínico Académico (XAN4Health) . TA XAN . 2019

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