Portal de investigação
In vivo systemic toxicity assessment of an oxidized dextrin-based hydrogel and its effectiveness as a carrier and stabilizer of granular synthetic bone substitutes
Data de publicação:
Autores da FMUP
Participantes de fora da FMUP
- Pereira, I
- Fraga, S
- Maltez, L
- Requicha, J
- Guardao, L
- Oliveira, J
- Prada, J
- Alves, H
- Santos, JD
- Teixeira, JP
- Pereira, JE
- Gama, FM
Unidades de investigação
Abstract
The worldwide incidence of bone disorders is raising, mainly due to aging population. The lack of effective treatments is pushing the development of synthetic bone substitutes (SBSs). Most ceramic-based SBSs commercially available display limited handling properties. Attempting to solve these issues and achieve wider acceptance by the clinicians, granular ceramics have been associated with hydrogels (HGs) to produce injectable/moldable SBSs. Dextrin, a low-molecular-weight carbohydrate, was used to develop a fully resorbable and injectable HG. It was first oxidized with sodium periodate and then cross-linked with adipic acid dihydrazide. The in vivo biocompatibility and safety of the dextrin-based HG was assessed by subacute systemic toxicity and skin sensitization tests, using rodent models. The results showed that the HG did not induce any systemic toxic effect, skin reaction, or genotoxicity, neither impaired the bone repair/regeneration process. Then, the HG was successfully combined with granular bone substitute, registered as Bonelike (250-500 mu m) to obtain a moldable/injectable SBS, which was implanted in tibial fractures in goats for 3 and 6 weeks. The obtained results showed that HG allowed the stabilization of the granules into the defect, ensuring effective handling, and molding properties of the formulation, as well as an efficient cohesion of the granules. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1678-1689, 2019.
Dados da publicação
- ISSN/ISSNe:
- 1549-3296, 1552-4965
- Tipo:
- Article
- Páginas:
- 1678-1689
- DOI:
- 10.1002/jbm.a.36683
- Link para outro recurso:
- www.scopus.com
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A John Wiley & Sons Inc.
Citações Recebidas na Web of Science: 8
Citações Recebidas na Scopus: 13
Documentos
- Não há documentos
Filiações
Keywords
- dextrin; injectable hydrogel; synthetic bone substitutes; bone regeneration; in vivo biocompatibility
Financiamento
Proyectos asociados
Effects of Xanthohumol on Metabolic Syndrome Progression (XAN4Health) - NCT03561116
Investigador Principal: Raquel Ângela Silva Soares Lino
Ensaio Clínico Académico (XAN4Health) . TA XAN . 2019
Citar a publicação
Pereira I,Fraga S,Maltez L,Requicha J,Guardao L,Oliveira J,Prada J,Alves H,Santos JD,Teixeira JP,Pereira JE,Soares R,Gama FM. <i>In vivo</i> systemic toxicity assessment of an oxidized dextrin-based hydrogel and its effectiveness as a carrier and stabilizer of granular synthetic bone substitutes. J. Biomed. Mater. Res. Part A. 2019. 107. (8):p. 1678-1689. IF:3,525. (2).
