Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori

Autores da FMUP
Participantes de fora da FMUP
- Ferreira, R
- Goncalves, RFS
- Pinheiro, AC
- Oleastro, M
- Wagemans, J
- Lavigne, R
- Azeredo, J
- Melo, LDR
Unidades de investigação
Abstract
Helicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. pylori strains and UV radiation. One phage was isolated and further characterized to assess potential phage-inspired therapeutic alternatives to H. pylori infections. HPy1R is a new podovirus prophage with a genome length of 31,162 bp, 37.1% GC, encoding 36 predicted proteins, of which 17 were identified as structural. Phage particles remained stable at 37 degrees C, from pH 3 to 11, for 24 h in standard assays. Moreover, when submitted to an in vitro gastric digestion model, only a small decrease was observed in the gastric phase, suggesting that it is adapted to the gastric tract environment. Together with its other characteristics, its capability to suppress H. pylori population levels for up to 24 h post-infection at multiplicities of infection of 0.01, 0.1, and 1 suggests that this newly isolated phage is a potential candidate for phage therapy in the absence of strictly lytic phages.
Dados da publicação
- ISSN/ISSNe:
- 1661-6596, 1661-6596
- Tipo:
- Article
- Páginas:
- 7885-
- DOI:
- 10.3390/ijms23147885
- Link para outro recurso:
- www.scopus.com
International Journal of Molecular Sciences Multidisciplinary Digital Publishing Institute (MDPI)
Citações Recebidas na Web of Science: 8
Citações Recebidas na Scopus: 9
Documentos
- Não há documentos
Filiações
Filiações não disponíveis
Keywords
- Helicobacter pylori; prophage; phage therapy; genomic analysis
Proyectos asociados
Unravelling Helicobacter pylori strategies to disrupt cell-cell junctions
Investigador Principal: Maria do Céu Fontes Herdeiro Figueiredo
Estudo Clínico Académico (Helicobacter) . 2019
Immune checkpoint inhibitors for the treatment of gastric cancer
Investigador Principal: Maria do Céu Fontes Herdeiro Figueiredo
Estudo Clínico Académico . 2021
Evaluation of the use of Helicobacter pylori genotypes and gastric microbiota markers to identify individuals at high risk of gastric carcinoma
Investigador Principal: Maria do Céu Fontes Herdeiro Figueiredo
Estudo Clínico Académico . 2021
Citar a publicação
Ferreira R,Sousa C,Goncalves RFS,Pinheiro AC,Oleastro M,Wagemans J,Lavigne R,Figueiredo C,Azeredo J,Melo LDR. Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori. Int. J. Mol. Sci. 2022. 23. (14):p. 7885-7885. IF:5,600. (1).