Portal de investigação
The Influence of Adipocyte Secretome on Selected Metabolic Fingerprints of Breast Cancer Cell Lines Representing the Four Major Breast Cancer Subtypes
Data de publicação:
Autores da FMUP
Participantes de fora da FMUP
- Luis, C
- Guerra Carvalho, B
- Braga, PC
- Guedes, C
- Patricio, E
- Alves, MG
- Fernandes, R
Unidades de investigação
Abstract
Molecular subtype (MS) is one of the most used classifications of breast cancer (BC). Four MSs are widely accepted according to receptor expression of estrogen, progesterone, and HER2. The impact of adipose tissue on BC MS metabolic impairment is still unclear. The present work aims to elucidate the metabolic alterations in breast cancer cell lines representing different MSs subjected to adipocyte associated factors. Preadipocytes isolated from human subcutaneous adipose tissue were differentiated into mature adipocytes. MS representative cell lines were exposed to mature adipocyte secretome. Extracellular medium was collected for metabolomics and RNA was extracted to evaluate enzymatic expression by RT-PCR. Adipocyte secretome exposure resulted in a decrease in the Warburg effect rate and an increase in cholesterol release. HER2+ cell lines (BT-474 and SK-BR-3) exhibited a similar metabolic pattern, in contrast to luminal A (MCF-7) and triple negative (TN) (MDA-MB-231), both presenting identical metabolisms. Anaplerosis was found in luminal A and TN representative cells, whereas cataplerotic reactions were likely to occur in HER2+ cell lines. Our results indicate that adipocyte secretome affects the central metabolism distinctly in each BC MS representative cell line.
Dados da publicação
- ISSN/ISSNe:
- 2073-4409, 2073-4409
- Tipo:
- Article
- Páginas:
- 2123-
- Link para outro recurso:
- www.scopus.com
Cells Multidisciplinary Digital Publishing Institute (MDPI)
Citações Recebidas na Scopus: 4
Documentos
- Não há documentos
Filiações
Keywords
- breast cancer; obesity; molecular subtype; cancer cell metabolism; Warburg effect; cell culture
Campos de estudo
Financiamento
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Citar a publicação
Luis C,Guerra B,Braga PC,Guedes C,Patricio E,Alves MG,Fernandes R,Soares R. The Influence of Adipocyte Secretome on Selected Metabolic Fingerprints of Breast Cancer Cell Lines Representing the Four Major Breast Cancer Subtypes. Cells. 2023. 12. (17):p. 2123-2123. IF:6,000. (2).
