ACE and ACE2 catalytic activity in the fecal content along the gut

Data de publicação: Data Ahead of Print:

Autores da FMUP

  • Teresa Maria De Jesus Teixeira De Sousa

    Autor

Participantes de fora da FMUP

  • Ferreira-Duarte, M
  • Oliveira, LCG
  • Quintas, C
  • Esteves-Monteiro, M
  • Duarte-Araújo, M
  • Casarini, DE
  • Morato, M

Unidades de investigação

Abstract

Background: Angiotensin-converting enzyme (ACE) and ACE2 are two major enzymes of the renin-angiotensin-aldosterone system (RAAS), which control the formation/degradation of angiotensin (Ang) II and Ang1-7, regulating their opposite effects. We aimed at evaluating the catalytic activity of ACE and ACE2 in the intestinal content and corresponding intestinal tissue along the gut of Wistar Han rats. Methods: Portions of the ileum, cecum, proximal colon, and distal colon, and the corresponding intestinal content were collected from Wistar Han rats. Enzyme activity was evaluated by fluorometric assays using different substrates: Hippuryl-His-Leu for ACE-C-domain, Z-Phe-His-Leu for ACE-N-domain, and Mca-APK(Dnp) for ACE2. ACE and ACE2 concentration was assessed by ELISA. Ratios concerning concentrations and activities were calculated to evaluate the balance of the RAAS. Statistical analysis was performed using Friedman test followed by Dunn's multiple comparisons test or Wilcoxon matched-pairs test whenever needed. Key Results: ACE and ACE2 are catalytically active in the intestinal content along the rat gut. The ACE N-domain shows higher activity than the C-domain both in the intestinal content and in the intestinal tissue. ACE and ACE2 are globally more active in the intestinal content than in the corresponding intestinal tissue. There was a distal-to-proximal prevalence of ACE2 over ACE in the intestinal tissue. Conclusions & Inferences: This work is the first to report the presence of catalytically active ACE and ACE2 in the rat intestinal content, supporting future research on the regulatory role of the intestinal RAAS on gut function and a putative link to the microbiome.

Dados da publicação

ISSN/ISSNe:
1365-2982, 1350-1925

Neurogastroenterology and Motility  Wiley-Blackwell Publishing Ltd

Tipo:
Article
Páginas:
-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 1

Citações Recebidas na Scopus: 3

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Keywords

  • angiotensin-converting enzyme (ACE); angiotensin-converting enzyme 2 (ACE2); intestine; renin-angiotensin-aldosterone system (RAAS); stools

Financiamento

Proyectos asociados

Unresolved inflammation and endothelitis in severe COVID-19 patients: identification of risk stratification biomarkers and therapeutic targets. (severe COVID-19)

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Estudo Clínico Académico (Severe COVID-19) . FCT . 2020

Endocan: a novel biomarker for risk stratification, prognosis, and therapeutic monitoring in human cardiovascular and renal diseases

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico (Endocan) . 2020

Renal renin-angiotensin system activation in acute heart failure and its association with endothelial dysfunction, inflammation and oxidative stress.

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico (Renal) . 2021

Urinary cysteinyl leukotrienes as biomarkers of endothelial activation, inflammation and oxidative stress and their relationship with organ dysfunction in human septic shock

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico . 2021

Evaluation of 11-dehydro-thromboxane B2 as a biomarker of severity and putative therapeutic target in human acute heart failure and cardiogenic shock

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico . 2022

Endothelial dysfunction in different stages of diseases severity of COVID-19: focus on endocan and arterial hypertension

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico . 2022

Evaluation of urinary cysteinyl leukotrienes as biomarkers of severity and putative therapeutic targets in COVID-19 patients

Investigador Principal: Teresa Maria de Jesus Teixeira de Sousa

Estudo Clínico Académico . 2022

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