Portal de investigação
Engineered liposomes to deliver nucleic acid mimics in Escherichia coli
Data de publicação:
Data Ahead of Print:
Autores da FMUP
Participantes de fora da FMUP
- Moreira, L
- Guimaraes, NM
- Pereira, S
- Santos, R.
- Loureiro, J.
- Ferreira, RM
- Pereira, M.
- Azevedo, N.
Unidades de investigação
Abstract
Antisense oligonucleotides (ASOs) composed of nucleic acid mimics (NAMs) monomers are considered as po-tential novel therapeutic drugs against bacterial infections. However, bacterial envelopes are generally imper-meable to naked oligonucleotides. Herein, liposomes loaded with NAMs-modified oligonucleotides (LipoNAMs) were evaluated to deliver ASOs in Escherichia coli. Specifically, we tested several surface modifications that included methoxyPEG conjugated to different lipid anchors or modification of the PEG distal ends with mal-eimide groups and antibodies. MethoxyPEG coated LipoNAMs showed low delivery efficiency for most bacteria, but maleimide-functionalized PEG LipoNAMs were able to deliver ASOs to nearly half of the bacterial population. Conjugation of antibodies to maleimide-functionalized PEG LipoNAMs increased 1.3-fold the delivery efficiency, enhancing the selectivity towards E. coli and biocompatibility. This work demonstrated for the first time that the coupling of antibodies to PEGylated liposomes can significantly improve the delivery of ASOs in E. coli, which might bring alternative routes for the treatment of bacterial infections in the future.
Dados da publicação
- ISSN/ISSNe:
- 1873-4995, 0168-3659
- Tipo:
- Article
- Páginas:
- 489-500
- Link para outro recurso:
- www.scopus.com
Journal of Controlled Release Elsevier
Citações Recebidas na Scopus: 6
Documentos
- Não há documentos
Filiações
Keywords
- Antisense oligonucleotides; Cationic liposomes; PEGylation; Immunoliposomes; Nucleic acid analogues; bacteria
Financiamento
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Citar a publicação
Moreira L,Guimaraes NM,Pereira S,Santos R,Loureiro J,Ferreira RM,Figueiredo C,Pereira M,Azevedo N. Engineered liposomes to deliver nucleic acid mimics in<i> Escherichia</i><i> coli</i>. J. Control. Release. 2023. 355. p. 489-500. IF:10,800. (1).
