Effects of NADPH Oxidase Isoform-2 (NOX2) Inhibition on Behavioral Responses and Neuroinflammation in a Mouse Model of Neuropathic Pain

Data de publicação:

Autores da FMUP

  • Teresa Maria De Jesus Teixeira De Sousa

    Autor

  • Dora Raquel Da Silva Pinho

    Autor

Participantes de fora da FMUP

  • Teixeira Santos, L
  • Verissimo, E
  • Martins, S
  • Albino-Teixeira, A

Unidades de investigação

Abstract

NADPH oxidase isoform-2 (NOX2) has been implicated in the pathophysiology of neuropathic pain (NP), mostly through the modulation of neuroinflammation. Since it is also accepted that some neuroimmune mechanisms underlying NP are sex-dependent, we aimed to evaluate the effects of early systemic treatment with the NOX2-selective inhibitor (NOX2i) GSK2795039 on behavioral responses and spinal neuroinflammation in spared nerve injury (SNI)-induced NP in male and female mice. Mechanical sensitivity was evaluated with the von Frey test, while general well-being and anxiety-like behavior were assessed with burrowing and light/dark box tests. Spinal microglial activation and cytokines IL-1 beta, IL-6, and IL-10, as well as macrophage colony-stimulating factor (M-CSF) were evaluated by immunofluorescence and multiplex immunoassay, respectively. NOX2i treatment reduced SNI-induced mechanical hypersensitivity and early SNI-induced microglial activation in both sexes. SNI-females, but not males, showed a transient reduction in burrowing activity. NOX2i treatment did not improve their burrowing activity, but tendentially reduced their anxiety-like behavior. NOX2i marginally decreased IL-6 in females, and increased M-CSF in males. Our findings suggest that NOX2-selective inhibition may be a potential therapeutic strategy for NP in both male and female individuals, with particular interest in females due to its apparent favorable impact in anxiety-like behavior.

Dados da publicação

ISSN/ISSNe:
2227-9059, 2227-9059

Biomedicines  MDPI AG

Tipo:
Article
Páginas:
416-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 1

Citações Recebidas na Scopus: 2

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Keywords

  • neuropathic pain; spared nerve injury; NOX2; GSK2795039; pain-related behavior; neuroinflammation; sex differences

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Estudo Clínico Académico . 2021

Evaluation of 11-dehydro-thromboxane B2 as a biomarker of severity and putative therapeutic target in human acute heart failure and cardiogenic shock

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Estudo Clínico Académico . 2022

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Estudo Clínico Académico . 2022

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Estudo Clínico Académico . 2022

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