Portal de investigação
Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
Data de publicação:
Autores da FMUP
Participantes de fora da FMUP
- Osorio, H
- Silva, C
- Ferreira, M
- Barros, R
- Mendonça, F
Unidades de investigação
Abstract
Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed following the single-pot, solid-phase-enhanced sample preparation approach-SP3 and enzymatic digestion with trypsin. The resulting peptides were analyzed by LC-MS Liquid Chromatography-Mass Spectrometry (LC-MS). The comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification (LFQ). A total of 6599 protein groups were identified in the 40 samples. Thirty-seven proteins were differentially expressed among the two groups, with 16 upregulated and 21 downregulated in the diabetic cohort. Statistical overrepresentation tests were considered for different annotation sets including the Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and Disease functional databases. Upregulated proteins in the GC samples from diabetic patients were particularly enriched in respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with epithelial cancers, intestinal diseases, and cell-cell junction cellular components. Taken together, these results support the data already obtained by previous studies that associate diabetes with metabolic disorders and diabetes-associated diseases, such as Alzheimer's and Parkinson's, and also provide valuable insights into seven GC-associated protein targets, claudin-3, polymeric immunoglobulin receptor protein, cadherin-17, villin-1, transglutaminase-2, desmoglein-2, and mucin-13, which warrant further investigation.
Dados da publicação
- ISSN/ISSNe:
- 2077-0383, 2077-0383
- Tipo:
- Article
- Páginas:
- 1-14
- DOI:
- 10.3390/jcm10030407
- Link para outro recurso:
- www.scopus.com
Journal of Clinical Medicine MDPI AG
Citações Recebidas na Web of Science: 27
Citações Recebidas na Scopus: 38
Documentos
- Não há documentos
Filiações
Keywords
- gastric cancer (GC); diabetes mellitus (DM); proteomics; label free quantification (LFQ)
Financiamento
Proyectos asociados
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Investigador Principal: Valdemar de Jesus Conde Máximo
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Gastric Cancer Morphological, Immunophenotypic and molecular heterogeneity
Investigador Principal: Maria de Fátima Machado Henriques Carneiro
Estudo Clínico Académico . 2020
Citar a publicação
Osorio H,Silva C,Ferreira M,Gullo I,Máximo V,Barros R,Mendonça F,Oliveira C,Carneiro F. Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus. J. Clin. Med. 2021. 10. (3):p. 1-14. IF:4,964. (2).
