Portal de investigação
Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series
Data de publicação:
Autores da FMUP
Participantes de fora da FMUP
- Macedo, S
- Pestana, A
- Santos, L
- Neves, C
- Guimaraes, S
- Duarte Neto, A
- Dolhnikoff, M
- Saldiva, P
- Alves, G
- Oliveira, R
- Cabanes, D
- Sobrinho Simoes, M
Unidades de investigação
Abstract
Objective: To understand whether thyroid cells can be directly infected by the SARS-CoV-2 virus and to establish a putative correlation with the expression of the host entry machinery: ACE-2, TMPRSS2, and furin. Methods: We assessed the presence of SARS-CoV-2 virus at the gene level by RT-PCR, viral RNA transcripts localization by in situ hybridization, and by detecting viral proteins by immunohistochemistry for the nucleocapsid and the spike proteins. Furthermore, we also described the immunoexpression of key host factors for virus entry in the COVID-19 thyroid samples. Results: We performed RT-PCR for SARS-CoV-2 in all autopsy specimens and detected viral genome positivity in 13 of 15 thyroid tissues and in a lung specimen. In 9 of the 14 positive samples, we were also able to confirm SARS-CoV-2 signal by in situ hybridization. Immunohistochemistry for the viral nucleocapsid and spike protein was also positive for ten and nine of the RT-PCR-positive cases, respectively, but revealed a lower sensitivity. We also described, for the first time in a COVID-19 series, the immunohistochemical expression of ACE-2, TMPRSS2, and furin in the thyroid. Conclusions: Our results obtained in thyroid specimens from deceased COVID-19 patients indicate that thyrocytes can be directly infected by SARS-CoV-2 since we detected the presence of SARS-CoV-2 genome in follicular cells. Nevertheless, we did not find a clear correlation between the presence of viral genome and the expression of the host factors for virus entry, namely ACE-2, TMPRSS2, and furin.
Dados da publicação
- ISSN/ISSNe:
- 2235-0640, 2235-0802
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.1530/ETJ-22-0074
- Link para outro recurso:
- www.scopus.com
European Thyroid Journal S. Karger AG
Citações Recebidas na Web of Science: 7
Citações Recebidas na Scopus: 9
Documentos
- Não há documentos
Filiações
Keywords
- SARS-CoV-2; thyroid; ACE-2; TMPRSS2; furin
Financiamento
Proyectos asociados
The role of hepatocyte apoptosis markers in predicting the histological and serological activity in steatohepatitis
Investigador Principal: Maria de Fátima Machado Henriques Carneiro
Estudo Clínico Académico . 2021
Gastric Cancer Morphological, Immunophenotypic and molecular heterogeneity
Investigador Principal: Maria de Fátima Machado Henriques Carneiro
Estudo Clínico Académico . 2020
Citar a publicação
Macedo S,Pestana A,Santos L,Neves C,Guimaraes S,Duarte A,Dolhnikoff M,Saldiva P,Alves G,Oliveira R,Cabanes D,Carneiro F,Sobrinho M,Soares Dias Ferreira A. Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series. Eur. Thyroid J. 2022. 11. (4):e220074. IF:4,700. (2).
