Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays

Data de publicação:

Autores da FMUP

  • Maria Do Céu Fontes Herdeiro Figueiredo

    Autor

  • Maria De Fátima Machado Henriques Carneiro

    Autor

  • Celso Albuquerque Reis

    Autor

Participantes de fora da FMUP

  • Silva, LM
  • Correia, VG
  • Moreira, ASP
  • Domingues, MRM
  • Ferreira, RM
  • Azevedo, N.
  • Marcos Pinto, R
  • Magalhaes, A
  • Feizi, T
  • Ferreira, JA
  • Coimbra, MA
  • Palma, AS

Unidades de investigação

Abstract

The structural diversity of the lipopolysaccharides (LPSs) from Helicobacter pylori poses a challenge to establish accurate and strain-specific structure-function relationships in interactions with the host. Here, LPS structural domains from five clinical isolates were obtained and compared with the reference strain 26695. This was achieved combining information from structural analysis (GC-MS and ESI-MSn) with binding data after interrogation of a LPS-derived carbohydrate microarray with sequence-specific proteins. All LPSs expressed Lewisx/y and N-acetyllactosamine determinants. Ribans were also detected in LPSs from all clinical isolates, allowing their distinction from the 26695 LPS. There was evidence for 1,3-D-galactans and blood group H-type 2 sequences in two of the clinical isolates, the latter not yet described for H. pylori LPS. Furthermore, carbohydrate microarray analyses showed a strain-associated LPS recognition by the immune lectins DC-SIGN and galectin-3 and revealed distinctive LPS binding patterns by IgG antibodies in the serum from H. pylori-infected patients.

Dados da publicação

ISSN/ISSNe:
1879-1344, 0144-8617

Carbohydrate Polymers  Elsevier Ltd.

Tipo:
Article
Páginas:
-
Link para outro recurso:
www.scopus.com

Citações Recebidas na Web of Science: 12

Citações Recebidas na Scopus: 16

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Keywords

  • Helicobacter pylori; Lipopolysaccharides; Mass spectrometry; Carbohydrate microarrays; Host immune receptors; Human sera

Financiamento

Proyectos asociados

Unravelling Helicobacter pylori strategies to disrupt cell-cell junctions

Investigador Principal: Maria do Céu Fontes Herdeiro Figueiredo

Estudo Clínico Académico (Helicobacter) . 2019

Gastric Cancer Morphological, Immunophenotypic and molecular heterogeneity

Investigador Principal: Maria de Fátima Machado Henriques Carneiro

Estudo Clínico Académico . 2020

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