Portal de investigação
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays
Data de publicação:
Autores da FMUP
Participantes de fora da FMUP
- Silva, LM
- Correia, VG
- Moreira, ASP
- Domingues, MRM
- Ferreira, RM
- Azevedo, N.
- Marcos Pinto, R
- Magalhaes, A
- Feizi, T
- Ferreira, JA
- Coimbra, MA
- Palma, AS
Unidades de investigação
Abstract
The structural diversity of the lipopolysaccharides (LPSs) from Helicobacter pylori poses a challenge to establish accurate and strain-specific structure-function relationships in interactions with the host. Here, LPS structural domains from five clinical isolates were obtained and compared with the reference strain 26695. This was achieved combining information from structural analysis (GC-MS and ESI-MSn) with binding data after interrogation of a LPS-derived carbohydrate microarray with sequence-specific proteins. All LPSs expressed Lewisx/y and N-acetyllactosamine determinants. Ribans were also detected in LPSs from all clinical isolates, allowing their distinction from the 26695 LPS. There was evidence for 1,3-D-galactans and blood group H-type 2 sequences in two of the clinical isolates, the latter not yet described for H. pylori LPS. Furthermore, carbohydrate microarray analyses showed a strain-associated LPS recognition by the immune lectins DC-SIGN and galectin-3 and revealed distinctive LPS binding patterns by IgG antibodies in the serum from H. pylori-infected patients.
Dados da publicação
- ISSN/ISSNe:
- 1879-1344, 0144-8617
- Tipo:
- Article
- Páginas:
- -
- Link para outro recurso:
- www.scopus.com
Carbohydrate Polymers Elsevier Ltd.
Citações Recebidas na Web of Science: 12
Citações Recebidas na Scopus: 16
Documentos
- Não há documentos
Filiações
Keywords
- Helicobacter pylori; Lipopolysaccharides; Mass spectrometry; Carbohydrate microarrays; Host immune receptors; Human sera
Financiamento
Proyectos asociados
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Citar a publicação
Silva LM,Correia VG,Moreira A,Domingues MRM,Ferreira RM,Figueiredo C,Azevedo N,Marcos R,Carneiro F,Magalhaes A,Reis C,Feizi T,Ferreira JA,Coimbra MA,Palma AS. <i>Helicobacter pylori</i> lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays. Carbohydr Polym. 2021. 253. 117350. IF:10,723. (1).
